SSCP: single strand conformation polymorphisms
DHPLC: denatured high performance liquid chromatography
In our lab,
SSCP is performed, using BioRad's DCode System.
DHPLC is performed, using Transgenomic's WAVE System.
| SSCP |
Forward
|
Reverse
|
||
| Exon |
Name
|
Sequence
|
Name
|
Sequence
|
| 2 |
Ex2F
|
TGG CAT GCC ATG CCG TCC TGA |
Ex2Q
|
CCC CAG CCA GGT AGT CCT GCA |
| 3 |
Ex3F
|
ACT GCT GCC TTC GAG TCC CAC |
Ex3R
|
TCT GCA GGC GGC CAG AGG CCT |
| 4 |
BW1a
|
CCG CCC GCG CTG TTC CAG GAC CAG GTA AGC |
Ex4R
|
AGA TAT GAG GCT CAG TGG AA |
| 4 |
BrvUp
|
CAG GAT CCG ATG TGG TGG TTC TGT GCT T |
Ex4R
|
AGA TAT GAG GCT CAG TGG AA |
| 5 |
B2aSF
|
CAA GCT TGG TTT CCT TCT TGA A |
B3R
|
CTG CAC ATT GCG GAG GTG GGA C |
| 5 |
B1F
|
CGT CTT TGA CAA CGC CAT CCG A |
B5R
|
TTG GAG GAG GAG GTG GAG AGG T |
| 5 |
B2fSF
|
GTG GTG GTG AAG ATG GAC AGT TC |
Ex5Q
|
CCC CTT GCT TTC CAA GCC TGT |
| 5 |
B2cSF
|
TGC GCA TCT TTG CCC ACA AGA T |
B6R
|
GGT GGA AGC ACG GAG GCC AGC A |
| 6 |
Ex6F
|
CTC CCA GCT TCT TTC ACC AGT |
B3SR
|
CTT CCC CCC TTT GTG TCC TCA |
| 7 |
B4SF
|
AAG CAG GAT CTC ATT GCT GTC |
B4SR
|
GGG GAG GTC CCC GAG TGC ACT |
| 8 |
Ex8F
|
GAT TGG TGG CCG TGT GTG TTA |
B5SR
|
AGA ACT GAG ACT GTT GTA GAT |
| 9 |
B6SF
|
GGA TTT CAG TAC AGA CGT TCT |
B6SR
|
GCA GCA GGT ACT GCC AGG ACT |
| 10 |
B7aSF
|
GCC TCC TTT CTA ACC AAG CTG |
Brv2dn
|
CTG GCT GAG CCA CGG TGG CTG GTA A |
| 10 |
Ex10B
|
GAT CAT TAC CAG CGG GCA CG |
Brv2dn
|
CTG GCT GAG CCA CGG TGG CTG GTA A |
| 10 |
B7aSF
|
GCC TCC TTT CTA ACC AAG CTG |
Ex10Q
|
GAT CTT CCT CCT TCA GGT ACC |
|
|
|
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|
|
|
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| DHPLC |
Forward
|
Reverse
|
||
| Exon |
Name
|
Sequence
|
Name
|
Sequence |
| 4 |
BW1A
|
CCG CCC GCG CTG TTC CAG GAC CAG GTA AGC |
BW1R
|
GCG CGC CGG CGG GGC TCC AAA TGG AGG GAG AG |
| 5 |
BW2aF
|
CCG CGG CAG CCT CCC TCC ACC ACC AA |
BW2aR
|
GGG CGG GGC ACT CCT CGC TGC TGG AG |
| 5 |
BW2bF
|
GCC CCG CCC ACC TCC TGG AGG ACC C |
BW2bR
|
CCG CGG GGG CTC GGC TCC AGG AAG ACA |
| 5 |
BW2CF
|
GGG GGC TCC GGT CCT GCT GGC CTC C |
BW2cR
|
CCG GCG CCC CCT TGC TTT CCA AGC C |
| 6 |
BW3F
|
CGC CCG GAG GAG GCT CCT TGG CT |
BW3R
|
CCC CGG TCT CAC TCC CTC CTT CCC C |
| 7 |
BW4F
|
GGC GCG GGT TCA GTT CAT CTT CTC C |
BW4R
|
CCC GCG CAC CTT GGG GTT TCC CT |
| 8 |
BW5F
|
GGC GCT GGG GGA TGT GGT GGG GCA G |
BW5R
|
GGC GGG GCG CAG CCA ATG GGC AGG |
| 9 |
BW6F
|
GCG GCG CCC TCT CCC CTG GGG ATT |
BW6R
|
GCA CCC CTA AGG GGA GGG CAG CAG G |
| 10 |
BW7F
|
CCG CGC CCG GCC CAC GTT CCT CTT GT |
BW7R
|
GGG GAC AAC AGC AGC AGG GTC AGA AGA |
Download primer sequences:
Contact: hamaguch@cshl.edu
Updated on April 13, 2002
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